Keratinocyte voltage and senescence

Evidence record

Source reviewed 2026-10-22 · Publication date retained

Original publication

2025-08-07 · PubMed records August 7 publication and September 19, 2025 eCollection. Do not relabel as 2026 research.

DOI

10.1016/j.isci.2025.113275

Authors

Hamid Sediqi, Michael Levin

Evidence class

Published experiment — human-derived cells in vitro

Source access

Publisher and supplementary PDF requests returned 403; PMC returned browser check. PubMed abstract, metadata and complete figure captions inspected. Donor/source hierarchy remains unverified.

Model

Human epidermal keratinocytes undergoing culture-associated senescence.

Donors / population

Count: UNKNOWN . Unknown; biological replicate counts must not be presented as donor counts.

Experimental units

Culture-level biological replicates; n differs by endpoint. Figure 1 reports n=6. Figure 5 cytokine tests report four biological replicates with technical triplicates.

Controls

Earlier culture time points, control medium, and hyperpolarizing/depolarizing comparisons; short post-treatment observation.

Endpoints

Voltage-sensitive fluorescence/lifetime, spatial organization, cell counts, senescence markers, chromatin condensation and cytokines.

Finding in scope

Senescence associated with depolarization and reduced spatial organization/responsiveness. Electrical perturbations changed selected phenotypes. Pinacidil SA-β-Gal was not significantly different from control (Figure 5).

Limitations

Culture phenotypes and dye-based measures do not establish human rejuvenation or durable tissue restoration. Drug specificity, donor generalizability and long-term safety require separate tests.

Next test

Audit source-cell and replicate provenance; test orthogonal voltage measurements, matched perturbation controls and donor-level replication with a prespecified functional endpoint and longer follow-up.

Human clinical evidence

None established by this study.

Translate evidence into a question.

This is external research. It is not a Karam result, clinical service or claim of an available intervention.