Tools / MEASURE
Microscopy scale check
Convert a pixel measurement using a known scale bar in the same image.
Illustrative example
The result
Method & assumptions
The scale bar and feature must be measured in the same, uniformly resized image. This does not correct perspective, optical distortion, or inconsistent magnification. Cropping is acceptable; unequal stretching is not.
Sanity check
A 200-pixel scale bar representing 50 µm gives 0.25 µm/pixel; an 80-pixel feature is 20 µm.
Scale = reference µm / reference pixels Feature length (µm) = measured pixels × scale
Implementation version 1.0 · 2026-10-15. Deterministic formulas or editorial rules, with no AI model or external service processing your inputs.