KARAMBIOSCIENCE
EST. 2026
EVIDENCE / 02
CRISPR / Research & discoveryOverviewTarget evidenceProgramsWork with KaramMethodsSources
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CRISPR Atlas / External evidence
Single-cell perturbation study

MitoPerturb-Seq: nuclear regulation of mitochondrial outcomes

MitoPerturb-Seq interrogates nuclear genes involved in mitochondrial DNA copy number and heteroplasmy at single-cell resolution. The measured response links nuclear perturbations with mitochondrial state.

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Concept illustration / not experimental evidence

What was reported

MitoPerturb-Seq interrogates nuclear genes involved in mitochondrial DNA copy number and heteroplasmy at single-cell resolution. The measured response links nuclear perturbations with mitochondrial state. [C13]

What the result does not establish

The method should not be described as routine direct mitochondrial DNA editing with ordinary Cas9 and a standard guide. Human therapeutic benefit is not established.

Measured endpoint

Mitochondrial DNA depletion, copy number and heteroplasmy responses.

Karam’s next question

Which mitochondrial functional outcome matters beyond copy number, and how should nuclear and mitochondrial interventions be distinguished?

Assessment-derived brief. Cited in the supplied 15 October 2026 project assessment; not independently reviewed in this website release. Karam has not reproduced the experiment or independently audited the underlying dataset. This is not Karam-generated evidence.

Record transparency

The details—and the missing values.

Extraction scope: supplied project assessment, not an independent full-methods extraction
Study date2026-04-01
Reported regionUnited Kingdom
Species / biological systemCell systems
Target / subjectNuclear regulators of mitochondrial DNA
Cell or population contextSingle-cell mitochondrial-response models
Perturbation directionGene-specific perturbation
MethodCRISPR screening / single-cell readout
Delivery settingNuclear perturbation; not ordinary direct mitochondrial Cas9 editing
ComparatorNot extracted in the supplied assessment; review the primary source before using this field.
Donor contextNot extracted in the supplied assessment; review the primary source before using this field.
Sample size / unitNot extracted in the supplied assessment; review the primary source before using this field.
Follow-upNot extracted in the supplied assessment; review the primary source before using this field.
Safety measurementsNot extracted in the supplied assessment; review the primary source before using this field.
Independent replicationNot established in the supplied assessment
Data accessNot extracted in the supplied assessment; review the primary source before using this field.

What would this evidence change?

Use the context in this record to define a bounded research or diligence question. A cited finding is not a validated Karam asset.

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