Off-target findings differ across cells and organs

Single-cell and in vivo profiling found heterogeneous off-target and translocation outcomes, including events obscured by bulk analysis. Some experiments deliberately used a promiscuous guide.

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Concept illustration / not experimental evidence

What was reported

Single-cell and in vivo profiling found heterogeneous off-target and translocation outcomes, including events obscured by bulk analysis. Some experiments deliberately used a promiscuous guide. [C06]

What the result does not establish

The work does not provide a universal error rate for every editor or clinical guide. Results depend on the guide, cell population, delivery and assay sensitivity.

Measured endpoint

Off-target edits and translocation profiles by cell and organ.

Karam’s next question

What events might the selected measurement strategy miss in the actual tissue and cell population of interest?

Assessment-derived brief. Cited in the supplied 15 October 2026 project assessment; not independently reviewed in this website release. Karam has not reproduced the experiment or independently audited the underlying dataset. This is not Karam-generated evidence.

Record transparency

The details—and the missing values.

Extraction scope: supplied project assessment, not an independent full-methods extraction
Study date	2026-08-26
Reported region	Not assigned
Species / biological system	Cells and mice
Target / subject	Guide- and tissue-specific loci
Cell or population context	Individual cells and mouse tissues
Perturbation direction	Off-target / translocation measurement
Method	Cas9 outcome profiling
Delivery setting	Experimental cell- and organ-specific contexts
Comparator	Not extracted in the supplied assessment; review the primary source before using this field.
Donor context	Not extracted in the supplied assessment; review the primary source before using this field.
Sample size / unit	Not extracted in the supplied assessment; review the primary source before using this field.
Follow-up	Not extracted in the supplied assessment; review the primary source before using this field.
Safety measurements	Study subject: unintended sequence changes and rearrangements; not a complete product safety certification.
Independent replication	Not established in the supplied assessment
Data access	Not extracted in the supplied assessment; review the primary source before using this field.

What would this evidence change?

Use the context in this record to define a bounded research or diligence question. A cited finding is not a validated Karam asset.

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