KARAMBIOSCIENCE
EST. 2026
EVIDENCE / 02
CRISPR / Research & discoveryOverviewTarget evidenceProgramsWork with KaramMethodsSources
Concept illustrations comparing gene regulation, epigenome, base and prime editing.
CRISPR Atlas / External evidence
Cells + animals

Prime assembly: large DNA changes remain context-specific

The assessment describes donor-directed annealing with prime editing for large genomic replacement. One reported application was targeted CAR insertion into primary human T cells, with efficiency up to 28.1%.

Compare in the Atlas
Concept illustration / not experimental evidence

What was reported

The assessment describes donor-directed annealing with prime editing for large genomic replacement. One reported application was targeted CAR insertion into primary human T cells, with efficiency up to 28.1%. [C09]

What the result does not establish

Cell-line maxima, primary-cell results and mouse delivery are different experiments. They cannot be combined into one general performance promise or a claim of age reversal.

Measured endpoint

Large-fragment integration, replacement and context-specific editing efficiency.

Karam’s next question

What is the useful output in the exact target cell type, and which unintended integration outcomes require measurement?

Assessment-derived brief. Cited in the supplied 15 October 2026 project assessment; not independently reviewed in this website release. Karam has not reproduced the experiment or independently audited the underlying dataset. This is not Karam-generated evidence.

Record transparency

The details—and the missing values.

Extraction scope: supplied project assessment, not an independent full-methods extraction
Study date2026-08-28
Reported regionSouth Korea
Species / biological systemHuman cells; experimental mouse context
Target / subjectTargeted integration sites
Cell or population contextPrimary human T cells and additional experimental systems
Perturbation directionTargeted integration / replacement
MethodPrime editing
Delivery settingDifferent delivery settings reported
ComparatorNot extracted in the supplied assessment; review the primary source before using this field.
Donor contextNot extracted in the supplied assessment; review the primary source before using this field.
Sample size / unitNot extracted in the supplied assessment; review the primary source before using this field.
Follow-upNot extracted in the supplied assessment; review the primary source before using this field.
Safety measurementsNot extracted in the supplied assessment; review the primary source before using this field.
Independent replicationNot established in the supplied assessment
Data accessNot extracted in the supplied assessment; review the primary source before using this field.

What would this evidence change?

Use the context in this record to define a bounded research or diligence question. A cited finding is not a validated Karam asset.

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