The CRISPR method layer connects a target, intervention direction, biological model and measured outcome. It includes non-CRISPR comparators so the methods are not confused.
Concept illustration / not experimental evidence
Scope: 26 records from the supplied assessment; two link to existing SRSF1 and ER-100 pages. This layer contains 2026 research and a labeled sponsor update. Earlier foundational and administrative sources are in the separate 45-reference register. Missing extraction fields remain missing; the interface does not turn them into zeroes.
A cDNA overexpression screen, not CRISPR, identified SRSF1. Reported cellular improvements and small aged-mouse results motivate a replication question.
Target / subject
SRSF1
Direction
Increased expression
Cell / setting
Human fibroblasts and aged mice
Boundary
CRISPRa reproduction would be a new hypothesis, not a result of the paper. Small survival cohorts do not establish human lifespan benefit.
2026-10-08 · United States
Early human intervention · OSK reprogramming — not CRISPR
The assessment records a sponsor report of tolerability observations through day 56 and preliminary visual-field improvements in two of three participants.
Target / subject
OCT4 / SOX2 / KLF4
Direction
Controlled expression
Cell / setting
Three participants with glaucoma in a sponsor update
Boundary
This is an uncontrolled, early sponsor update, not confirmatory efficacy evidence or CRISPR-Cas9 editing. Trial status must be checked directly before any participation inquiry.
2026-09-30 · China
Plants + field trials · Cas9 validation + overexpression
Cas9 knockout lines helped validate a mechanism linking OsRLK with root-associated microbes and nitrogen-use efficiency. The beneficial yield-related field results came from overexpression lines.
Target / subject
OsRLK
Direction
Knockout for mechanism; overexpression for beneficial traits
Cell / setting
Root microbiome and nitrogen-use context
Boundary
Calling the favorable field result a successful knockout would reverse the intervention. Better nutrient use alone does not quantify avoided nitrous oxide emissions.
Structure-guided engineering produced a compact DNA-binding system and epigenetic regulation in human cells, with a silencing application in a mouse hepatitis B model. The central contribution is regulator size and control.
Target / subject
xCas12m / experimental silencing targets
Direction
Gene regulation / silencing
Cell / setting
Human-cell experiments; mouse hepatitis B model
Boundary
The system is Cas12m, not Cas9. A successful viral-disease model does not establish delivery or rejuvenation in aged muscle, brain or other tissues.
2026-09-16 · China
Plant mechanistic study · Regulatory mechanism study
The assessment describes donor-directed annealing with prime editing for large genomic replacement. One reported application was targeted CAR insertion into primary human T cells, with efficiency up to 28.1%.
Target / subject
Targeted integration sites
Direction
Targeted integration / replacement
Cell / setting
Primary human T cells and additional experimental systems
Boundary
Cell-line maxima, primary-cell results and mouse delivery are different experiments. They cannot be combined into one general performance promise or a claim of age reversal.
A phase 1a report described one-year durability of ANGPTL3 and lipid reductions after CTX310. The assessment notes that the abstract reported no treatment-related dose-limiting toxic effects.
Target / subject
ANGPTL3
Direction
Disease-related target editing
Cell / setting
Participants in an early-phase cardiovascular-risk program
Boundary
Biomarker reductions do not establish fewer cardiovascular events or longer life. A small early-phase safety report is not a guarantee of long-term product safety.
Single-cell and in vivo profiling found heterogeneous off-target and translocation outcomes, including events obscured by bulk analysis. Some experiments deliberately used a promiscuous guide.
Target / subject
Guide- and tissue-specific loci
Direction
Off-target / translocation measurement
Cell / setting
Individual cells and mouse tissues
Boundary
The work does not provide a universal error rate for every editor or clinical guide. Results depend on the guide, cell population, delivery and assay sensitivity.
2026-08-20 · Not assigned
Computational + experimental data · Long-read outcome analysis
CRISPRLungo analyzes long-read amplicon data to distinguish edited alleles and structural outcomes such as larger deletions and inversions. The author-lab description includes error correction, statistical filtering and a browser application.
Target / subject
Edited alleles and structural outcomes
Direction
Measurement / allele classification
Cell / setting
Long-read amplicon sequencing datasets
Boundary
A targeted amplicon assay does not establish that the whole genome is free of unintended changes. Karam has not independently validated this pipeline or adopted it as a commercial service.
Virus-like particle tools enabled targeted engineering and pooled screening of primary human myeloid cells. In the macrophage context highlighted in the assessment, disrupting TNFAIP3 promoted inflammation.
Target / subject
TNFAIP3 and other regulators
Direction
Disruption; context-dependent inflammation
Cell / setting
Monocytes, macrophages and dendritic cells
Boundary
A gene name is not an intervention recommendation. An effect can have the opposite direction from what another cell type or disease context might suggest.
2026-08-14 · Not assigned
Theoretical modeling · Synthetic horizontal transfer model
The assessment describes theoretical work on synthetic horizontal gene transfer in microbial communities, including possible dryland water-retention benefits.
Target / subject
Microbial community properties
Direction
Horizontal transfer / ecosystem model
Cell / setting
Theoretical restoration and dryland water-retention scenarios
Boundary
Horizontal transfer differs from biased parent-to-offspring inheritance. The paper is not evidence of a Cas9 climate product, safe field deployment or durable carbon removal.
OptiPrime combines mechanistic modeling with machine learning to rank prime-editing strategies. The assessment reports prospective evaluation in disease-relevant settings, including primary cells.
Target / subject
Design-specific prime-editing targets
Direction
Prediction / candidate ranking
Cell / setting
Several disease-relevant settings, including primary cells
Boundary
Predicted performance is not a safety certification. Commercial reuse requires a separate rights assessment; the publisher states that the code and server are for noncommercial use.
Screens identified Dusp5 and Zfp219 as regulators of T-cell expansion or cytotoxic function. Zfp219 loss combined with checkpoint inhibition improved tumor control in aged mice; the human ZNF219 observations were associative.
Target / subject
Dusp5 / Zfp219; human ZNF219
Direction
Loss of function; combination with checkpoint inhibition
Cell / setting
T cells in an aged tumor environment
Boundary
Mouse tumor control is not proof of general human immune rejuvenation. Dusp5, Zfp219 and human ZNF219 cannot be used interchangeably.
A focused Cas9 screen identified GNPTAB as a regulator of senescence. The assessment describes a noncanonical interaction with STING and a binding-deficient mutant that retained the tested lysosomal functions without inducing senescence.
This is mechanistic human-cell evidence, not treatment of people. Deleting or suppressing GNPTAB everywhere would not follow from an interaction-specific result.
2026-06-19 · Japan
Plant experimental study · TALE base editing — not CRISPR
A TALE-based chloroplast editor improved photosynthetic performance and plant growth in Arabidopsis. Its targeting system is not a guide-RNA-directed CRISPR platform.
Target / subject
Chloroplast Rubisco
Direction
Chloroplast sequence editing
Cell / setting
Photosynthesis and plant-growth models
Boundary
Faster plant growth is not evidence of durable atmospheric carbon removal. The molecular modality should not be mislabeled simply because both approaches edit genomes.
The assessment reports improved prime editing using lipid nanoparticles, including correction in a mouse phenylketonuria model. Delivery and RNA cargo design materially affected outcomes.
Target / subject
Disease-specific sequence targets
Direction
Sequence correction
Cell / setting
Mouse liver, including a phenylketonuria model
Boundary
Performance in mouse liver is not a demonstration of delivery to every organ, and does not establish treatment of normal aging in people.
2026-05-26 · Not assigned
Cage experiments + modeling · Population gene drive
The assessment describes a self-limiting gene-drive proof of principle with temporary mosquito population suppression in cages, supported by modeling.
Target / subject
Inheritance system; no design details provided here
Direction
Temporary population suppression
Cell / setting
Controlled mosquito cage populations
Boundary
Cage behavior does not establish reduced human disease, safe environmental release or a deployable climate product. No construction or release guidance is provided on this site.
Researchers at the University of Hyderabad disrupted the TOP2B promoter in HEK293T cells and observed senescence. It is a useful counterexample to the idea that removing gene activity necessarily rejuvenates cells.
Target / subject
TOP2B promoter
Direction
Promoter deletion
Cell / setting
HEK293T cells
Boundary
HEK293T is an immortalized laboratory model, not normal aged human tissue. The finding is a directional warning, not a validated human-aging target.
A CRISPRi screen of approximately 45,000 guides identified determinants of methane-dependent growth and informed improved culture productivity.
Target / subject
Methane-dependent growth regulators
Direction
Gene repression screen
Cell / setting
Methane-consuming bacterial culture
Boundary
The study does not demonstrate low-cost industrial treatment of ambient atmospheric methane or a net greenhouse-gas benefit. Productivity, methane removal and life-cycle impact are different outcomes.
A modeling study examined how dormant seed banks can delay spread and change predicted outcomes for plant gene drives.
Target / subject
Seed dormancy and inheritance
Direction
Model parameter comparison
Cell / setting
Dormant seed-bank scenarios
Boundary
The work is modeling, not a field result or demonstrated climate intervention. A simple inheritance expectation does not determine ecosystem-scale behavior.
2026-04-01 · United Kingdom
Single-cell perturbation study · CRISPR screening / single-cell readout
MitoPerturb-Seq interrogates nuclear genes involved in mitochondrial DNA copy number and heteroplasmy at single-cell resolution. The measured response links nuclear perturbations with mitochondrial state.
Target / subject
Nuclear regulators of mitochondrial DNA
Direction
Gene-specific perturbation
Cell / setting
Single-cell mitochondrial-response models
Boundary
The method should not be described as routine direct mitochondrial DNA editing with ordinary Cas9 and a standard guide. Human therapeutic benefit is not established.
Activating endogenous POLR2A with CRISPRa alleviated senescent phenotypes in the reported system. The assessment describes a pathway connecting LMO7-mediated regulation with MDM4, p53 and p21.
Target / subject
LMO7 / POLR2A / MDM4-p53-p21
Direction
Activation
Cell / setting
Senescence-associated cellular model
Boundary
This does not show that broad suppression of protective p53 signaling is a healthy-aging strategy. Specific context, identity and growth safeguards must remain visible.
A sorghum regulatory map measured more than 30,000 CRISPR-accessible mutations in reporter assays. The output highlighted in the assessment was changed expression of photosynthesis-related proteins.
Target / subject
Photosynthesis-related cis-regulatory sites
Direction
Regulatory variation / expression modulation
Cell / setting
Plant reporter-assay context
Boundary
A percentage change in protein expression is not the same percentage change in crop yield or carbon removal. Carbon-storage durability was not established by this readout.
Abasic modifications inspired by bacterial CRISPR RNAs reduced off-target cleavage while retaining useful on-target activity in the reported experimental systems.
Target / subject
SpCas9 guide / experimental loci
Direction
Guide modification
Cell / setting
Experimental cells; wider tissue performance not established
Boundary
Reduced off-target activity is not zero off-target activity, and this is not general human safety evidence. The paper is earlier-2026 context, not a last-three-months finding.
The study projected increased malaria burden under specified climate and present-control assumptions, with disruption from extreme weather contributing to the modeled burden.
Target / subject
Malaria burden under climate scenarios
Direction
Scenario comparison
Cell / setting
Africa under a specified climate scenario and control assumptions
Boundary
A projection is not evidence that a gene drive works. Disease resilience is also not greenhouse-gas removal or planetary cooling.
2026-01-26 · Not assigned
Greenhouse plant experiment · Transgenic overexpression — not CRISPR
The assessment reports 38% and 58% lower cumulative methane over 70 days for two transgenic rice genotypes. One genotype also showed adverse seed-production traits.
Target / subject
Rhizosphere hydrogen-cycling relationships
Direction
Overexpression
Cell / setting
Two transgenic rice genotypes in greenhouse conditions
Boundary
This used overexpression rather than CRISPR. Greenhouse emissions reductions do not establish field-scale agronomic performance or a commercially useful net climate outcome.
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Source trail
Scientific summaries are adapted from the supplied 15 October 2026 CRISPR Project Assessment. Source records show the original references and review access; this release is not a new systematic review.
